Summary
Type: SPE
Application #: 301540
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
C18 ec: 2 column vol. MeOH, then 2 column vol. dist. H2O
NH2: 1 ml ethyl acetate, then 1 ml dichloromethane; take care, that the column
does not run dry
Sample application:
Slowly pour 40 ml sample through the C18 ec column
Column washing:
5 ml water; dry C18 ec column 5 min under vacuum, rinse with 1 ml
dichloromethane, place C18 ec column onto NH2 column
Elution:
1 ml ethyl acetate through both columns
Authors: Macherey-Nagel
Source: LC Department